Description
Perfinity Workstation, developed in partnership with Perfinity Biosciences Inc., is a multi-column apparatus that automates protein separations and mass spec sample preparation for more efficient LC/MS analysis. Unlike 2D LC systems, this platform utilizes the concept of ‘digital elution’ to link various sample preparation processes. Targeted analytes bound in one column are forwarded on to the next process.
The Perfinity Workstation uses utilizes Shimadzu hardware components in conjunction with five Perfinity Optimized columns that each perform a step of the mass-spec sample-preparation process -affinity selection, buffer exchange, digestion, desalting and reverse-phase separation. The automated integration of these steps enables users to start with serum and have peptides ready for LC/MS analysis in 10 minutes. The automated process removes much of the equipment and labor associated with mass-spec analyses of proteins.
Key benefits include:
- Serum to purified peptides in < 10="" min.="" />
- Hands free operation – fully automated
- Significant reduction in cost per sample
- Easily customizable
The major advantage of the Perfinity Workstation’s sample-preparation approach is that users are not required to immobilize antibodies. Instead, scientists can add antibodies directly to the sample. By removing the immobilization process, users have eliminated a potential source of error, making LC/MS analysis with Perfinity Workstation more accurate and reliable.
This platform is customizable across several application areas, including assay development, protein purification, drug development and biomarker discovery.
*Available in the U.S. only